Promotion of direct somatic embryogenesis of <Emphasis Type="Italic">Oncidium</Emphasis> by adjusting carbon sources |
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Authors: | P I Hong J T Chen W C Chang |
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Institution: | (1) Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China;(2) Institute of Biotechnology, National University of Kaohsiung, Kaohsiung 811, Taiwan, Republic of China |
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Abstract: | To further optimize a culture medium for induction of direct embryo formation of Oncidium cvs. Gower Ramsey and Sweet Sugar, five kinds of carbon sources, cellibiose, fructose, glucose, maltose and sucrose at 10,
20, 30 and 60 g dm−3 were tested in this study. Cellibiose supply had an inhibitory effect and resulted in high percentage of explant browning
in both cultivars. By contrast, fructose, glucose and sucrose were all effective for direct embryo induction. In cv. Gower
Ramsey, the suitable ranges of concentration were found at 30–60 g dm−3 of sucrose, 10–20 g dm−3 of glucose and 20–30 g dm−3 of fructose, respectively. The suitable ranges for cv. Sweet Sugar were at 20–60 g dm−3 of sucrose, 10–30 g dm−3 of glucose, 10–20 g dm−3 of fructose and 30–60 g dm−3 of maltose, respectively. The highest amount of embryos was obtained at 30 g dm−3 of sucrose for cv. Gower Ramsey and at 20 g dm−3 of glucose for cv. Sweet Sugar. |
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Keywords: | Additional" target="_blank">Additional embryogenic response leaf explant orchid |
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