Chitinase isozymes induced by TYMV and <Emphasis Type="Italic">Leptosphaeria maculans</Emphasis> during compatible and incompatible interaction with <Emphasis Type="Italic">Brassica napus</Emphasis> |
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Authors: | B Profotová L Burketová O Valentová |
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Institution: | 1.Institute of Chemical Technology Prague,Prague,Czech Republic;2.Institute of Experimental Botany CAS,Prague,Czech Republic |
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Abstract: | Accumulation of extracellular chitinases in Brassica napus plants infected with Turnip yellow mosaic virus (TYMV) and fungal pathogen Leptosphaeria maculans was studied in both compatible and incompatible interaction. Analysis of apoplast fluid by means of non-denaturing anodic
and cathodic PAGE followed by in-gel detection of chitinase activity revealed a number of chitinase isozymes. TYMV induced
8 acidic and 4 basic isozymes in a systemic way. Except for one acidic and one basic isozyme, all other chitinases were also
constitutively present in low amounts in mock inoculated control. In TYMV systemically infected plants, chitinases were detected
in leaves expressing symptoms as well as in symptomless ones. Both virulent and avirulent L. maculans isolates induced production of chitinase isozymes in cotyledons in a time dependent manner. Some of them were present in
plants constitutively and their content increased after inoculation. Three of five acidic and two of three basic isozymes
responded to L. maculans infection. Chitinases started to accumulate before symptom appearance. First two acidic isozymes were detected 24 h after
inoculation. The difference between compatible and incompatibe interaction reflected two basic isozymes. |
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