Cytological studies on meiosis and male gametophyte development in autotetraploid cucumber |
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Authors: | W -P Diao S -Y Bao B Jiang L Cui C -T Qian and J -F Chen |
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Institution: | (1) State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agriculture University, 210095 Nanjing, China; |
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Abstract: | With improved staining and chromosome preparation techniques, meiosis of pollen mother cells (PMCs) and male gametophyte development
in autotetraploid cucumber (Cucumis sativus L.) was studied to understand the correlation between chromosomes behaviour and fertility. Various chromosome configurations,
e.g. multivalent, quadrivalents, trivalents, bivalents and univalents were observed in most PMCs at metaphase I. Lagging chromosomes
were frequently observed at anaphase in both meiotic divisions. In addition, chromosomes segregations were not synchronous
and equal in some PMCs during anaphase II and telophase II. Dyads, triads, tetrads with micronuclei and polyads were observed
at tetrad stage, and the frequencies of normal tetrad with four microcytes were only 55.4 %. The frequency of abnormal behaviour
in each stage of meiosis was counted, and the average value was 37.2 %. The normal meiotic process could be accomplished to
form the microspore tetrads via simultaneous cytokinesis. Most microspores could develop into fertile gametophytes with 2 cells and 3 germ pores through
the following stages: single-nucleus early stage, single-nucleus late stage and 2-celled stage. The frequency of abnormalities
was low during the process of male gametophyte development. The germination rate of pollen grains was 46.9 %. These results
suggested that abnormal meiosis in PMCs was the reason for low pollen fertility in the autotetraploid cucumber. |
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