Chloroplast-specific universal primers and their uses in plant studies |
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Authors: | Email author" target="_blank">N?HaiderEmail author |
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Institution: | 1.Department of Molecular Biology and Biotechnology,Atomic Energy Commission of Syria,Damascus,Syria |
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Abstract: | Universal (consensus) primers are those primers that have the ability to amplify the targeted region of DNA across a broad
range of individuals in a certain group of organisms. In plants, such universal primers have been designed to target regions
in the nuclear, mitochondrial or chloroplast genome. Among these three genomes, the chloroplast genome is the most suited
for the design of consensus primers due to the lower rate of evolution and hence conservation of gene order and sequence of
the genome among the different plant species compared to the other two genomes. Several molecular studies in plants have developed
and used chloroplast-specific universal primers. In this review, I present some examples of the nuclear DNA-specific universal
primers and discuss the features of the chloroplast DNA that make it the most suited for the design of such primers. I then
refer to all chloroplast-specific primers developed so far and provide some examples of molecular studies and applications
that made use of them. |
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Keywords: | |
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