A modified low copy number binary vector pUN for <Emphasis Type="Italic">Agrobacterium</Emphasis>-mediated plant transformation |
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Authors: | E Vaculková J Morav?íková I Matu?íková M Bauer J Libantová |
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Institution: | (1) Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, P.O. Box 39A, SK-95007 Nitra, Slovak Republic;(2) Research Institute of Animal Production, Hlohovska 2, SK-94992 Nitra, Slovak Republic |
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Abstract: | A modified low copy number plant binary vector pUN has been constructed and successfully used to clone unstable DNA sequences.
The vector pUN comprises of low copy number, broad host range RK2 replicon from pBin19 and of multiple cloning site (MCS)
and T-DNA region, both from a pBINPLUS-derived pLV06 vector. The absence of the ColE1 replicon in the backbone of the binary
vector significantly contributed to stability of hardly clonable DNA sequences and enabled their transfer into the tobacco
plants through Agrobacterium-mediated transformation. |
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Keywords: | ColE1 replicon Ti vector unstable DNA sequences |
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