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Optimization of Algerian fir somatic embryos maturation
Authors:B Vooková  J Machava  A ?algovi?ová and A Kormu?ák
Institution:(1) Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademick? 2, P.O. Box 39A, 950 07 Nitra, Slovak Republic
Abstract:The effect of maturation pretreatment on development and growth of Abies numidica De Lann. somatic embryos was studied. The most beneficial was pre-culturing on Schenk and Hildebrandt medium without growth regulator for 2 weeks. Dry mass accumulation of emblings was lower than that of seedlings after 50 d of culturing. Contents of microelements in seedlings were higher than in emblings, but macroelements contents were higher in emblings. Contents of chlorophyll a and chlorophyll b in cotyledons were higher in seedlings than in emblings while no qualitative differences were detected between the protein profiles of seedlings and emblings.
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