Optimization of Algerian fir somatic embryos maturation |
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Authors: | B Vooková J Machava A ?algovi?ová and A Kormu?ák |
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Institution: | (1) Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademick? 2, P.O. Box 39A, 950 07 Nitra, Slovak Republic |
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Abstract: | The effect of maturation pretreatment on development and growth of Abies numidica De Lann. somatic embryos was studied. The most beneficial was pre-culturing on Schenk and Hildebrandt medium without growth
regulator for 2 weeks. Dry mass accumulation of emblings was lower than that of seedlings after 50 d of culturing. Contents
of microelements in seedlings were higher than in emblings, but macroelements contents were higher in emblings. Contents of
chlorophyll a and chlorophyll b in cotyledons were higher in seedlings than in emblings while no qualitative differences were detected between the protein
profiles of seedlings and emblings. |
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