Propagation of Citrus reticulata via in vitro Seed Germination and Shoot Cuttings |
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Authors: | AM Hassanein MM Azooz |
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Institution: | (1) Botany Department, Faculty of Science, South Valley University, Sohag, 82524, Egypt;(2) Botany Department, Faculty of Science, South Valley University, Qena, 83523, Egypt |
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Abstract: | Seeds of Citrus reticulata were germinated efficiently when they were sown directly after their extraction from fruits harvested in January, and incubated
at constant temperature (25 °C). Seed drying decreased both the percentage of seed germination and the number of seedling
per seed. Germination of seeds was better on Murashige and Skoog (MS) medium supplemented with 0.5 mg dm−3 benzylaminopurine (BAP) than in a soil. Shoot cuttings obtained from germinated seeds were subcultured on B5 medium supplemented
with 1 mg dm−3 BAP, 0.5 mg dm−3 kinetin (KIN) and 0.5 mg dm−3 naphthalene acetic acid (NAA), where shoots grew and multiplied. They were rooted on half strength MS medium supplemented
with 0.25 mg dm−3 BAP, 0.5 mg dm−3 NAA and 1 mg dm−3 isobutyric acid (IBA). Rooting under light was better than under dark. Seedlings and shoot cuttings with roots were transferred
successfully to the soil after three weeks of acclimatization.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | mandarin phytohormones seed germination tissue culture |
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