Polypeptide markers for low temperature stress during seed germination in sunflower |
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Authors: | A Kumar S C Bhatla |
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Institution: | (1) Department of Botany, University of Delhi, Delhi, 110007, India |
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Abstract: | Sunflower seeds behaved as chilling and freezing sensitive and also exhibited acclimation under low seed moisture content
(< 1 %). At high seed moisture content (approx. 22 %) they tolerated chilling stress but failed to acclimate under freezing
temperatures. Pre-imbibitional chilling (5 °C) or freezing (−5 or −10 °C) stress significantly enhanced total soluble protein
(TSP) content. Chilling treatment after imbibition (in contrast to pre-imbibition) enhanced germination and this was accompanied
by increase in 30, 24 and 21.9 kDa TSPs content (3 d after germination). Freezing at −5 and −10 °C suppressed seed germination
and increased content of 78 and 56.2 kDa wall bound proteins. Chilling acclimation decreased 35.4, 33.9, 29.5, 23.4 and 21.4
kDa TSPs. |
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Keywords: | chilling stress freezing stress Helianthus annuus L protein |
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