Utilization of mercapto-2-ethanol as medium reductant for determination of the metabolic response of methanogens towards inorganic sulfur compounds |
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Authors: | L Bhatnagar M Henriquet JG Zeikus J-P Aubert |
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Institution: | Unitéde Physiologie Cellulaire, Département de B.G.M., Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris Cédex 15, France |
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Abstract: | Abstract Mercapto-2-ethanol was examined as a nontoxic and non-metabolizable reducing agent for growth of methanogens. It was used as a medium reductant to prove that Methanobacterium thermoautotrophicum and Methanobacterium strain ivanov grew with either sulfide or elemental sulfur as the sole source of nutrient sulfur but not with sulfate, thiosulfate, sulfite or dithionite. The later inorganic sulfur sources, except sulfate, were potent inhibitors of growth and methanogenesis at 5 mM. The practical utility of mercapto-2-ethanol as a reducing agent and the toxicity of inorganic sulfur sources on metabolic activity of the methanogens are discussed. |
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Keywords: | Methanobacterium thermoautotrophicum strain ΔH Methanobacterium strain ivanov practical reducing agent sulfite thiosulfate toxicity elemental sulfur as sulfur source inorganic sulfur metabolism |
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