Analysis of S-layer proteins of Lactobacillus brevis |
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Authors: | Tetsuji Yasui Kumiko Yoda Takayuki Kamiya |
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Institution: | Brewing Research Laboratories, Kirin Brewery Co., Ltd., Tsurumi-ku, Yokohama, 230, Japan |
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Abstract: | Abstract The presence of S-layer proteins in Lactobacillus brevis was examined by SDS-PAGE analysis. Thirty six out of a total of 41 L. brevis strains possessed S-layer proteins of molecular masses ranging from 38 to 55 kDa. Western blot analysis using antisera raised against whole cells of S-layer protein-carrying strains demonstrated the heterogeneity of L. brevis S-layer proteins. No clear relationship was observed between the presence of S-layer proteins or their immunological characteristics and the physiological activity of L. brevis as a beer spoilage organism. |
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Keywords: | Lactobacillus brevis S-layer Immunological characteristics |
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