Transformation of group A streptococci by electroporation |
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Authors: | Alexander Suvorov Jan Kok Gerard Venema |
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Institution: | Institute of Experimental Medicine, Leningrad, U.S.S.R.;Institute of Genetics, University of Groningen, Haren, The Netherlands |
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Abstract: | Abstract The introduction, via electroporation, of free plasmid DNA into three strains of Streptococcus pyogenes is described. The method is very simple and rapid and efficiencies vary from 1 × 103 to 4 × 104 per μg of DNA. The method was also used to introduce an integrative plasmid and transformants were obtained, albeit at a somewhat lower frequency (2 × 102). Some of the plasmids used in this study are derivatives of the Lactococcus lactis subsp. cremoris Wg2 plasmid pWV01. These broad host range vectors replicate in Gram-positives as well as Gram-negatives (viz. Escherichia coli ). Here we show that they also replicate in S. pyogenes and S. sanguis . |
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Keywords: | Streptococcus pyogenes Transformation Electroporation Plasmid |
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