13C-NMR studies on the influence of pH and nitrogen source on polyol pool formation in Aspergillus nidulans |
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| Authors: | Cor Dijkema Robert Pels Rijcken Harry CM Kester Jaap Visser |
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| Institution: | Department of Molecular Physics, Agricultural University, Generaal Foulkesweg 53, 6703 BM Wageningen, The Netherlands;Department of Genetics, Agricultural University, Generaal Foulkesweg 53, 6703 BM Wageningen, The Netherlands |
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| Abstract: | Abstract The composition of the polyol pools in Aspergillus nidulans mycelium during active growth on sucrose depends strongly on pH. At pH 2.5, only mannitol is present. A comparison between nitrate- and ammonium-grown cultures shows stimulation of the arabitol content with nitrate a former nitrogen source. When starved mycelium is incubated either with natural-abundance or 13C-enriched glucose, label appears rapidly in mannitol and arabitol, regardless of the nitrogen source or the pH used. |
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| Keywords: | Mannitol arabitol erythritol glycerol |
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