Small cryptic plasmids of Streptococcus pneumoniae belong to the pC194/pUB110 family of rolling circle plasmids |
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Authors: | Cordelia Schuster Mark van der Linden Regine Hakenbeck |
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Institution: | University of Kaiserslautern, Department of Microbiology, Paul Ehrlich Strasse, Geb. 23, D-67663 Kaiserslautern, Germany;Max-Planck Institut für Molekulare Genetik, Berlin, Germany |
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Abstract: | The DNA sequences of two related plasmids pPR1 and pPR3 described previously in Streptococcus pneumoniae isolates from Germany and Spain were now determined. Both plasmids belong to a family of rolling circle (RC) plasmids found in a variety of bacteria. Their GC content with 32% is lower than that of the S. pneumoniae chromosomal DNA. The plasmid pPR3 has a molecular size of 3160 bp with four putative open reading frames, whereas pPR1 contained a deletion of 313 bp that included the 5′-part of ORF2 and upstream regions and differed by three bp from pPR3. The predicted protein of ORF1 showed high similarity to replication proteins of RC plasmids with 74% identical amino acids to RepA of Streptococcus thermophilus plasmids. Sequences similar to the plus origin of replication of ssDNA plasmids were present in both plasmids. They also contained a 152-bp region with over 83% identity to the minus origin of replication of the Streptococcus agalacticae plasmid pMV158. |
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Keywords: | Streptococcus pneumoniae Single stranded DNA plasmid Rep protein Single-strand origin |
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