Saccharides of seven Pseudomonas aeruginosa immunotypes |
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| Authors: | Eugeny S Stanislavsky Elena V Kholodkova Yuriy A Knirel Nina A Kocharova |
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| Institution: | I.I. Mechinikov Central Research Institute of Vaccines and Sera, Ministry of Health of the U.S.S.R., U.S.S.R.;N.D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the U.S.S.R., Moscow, U.S.S.R. |
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| Abstract: | Abstract The structures of O-specific polysaccharides obtained by mild acid degredation of lipopolysaccharides (LPS) from seven Pseudomonas aeruginosa Fisher's immunotypes have been studied. The polysaccharides consist mainly of monoamino and diamino sugars, frequently also carrying acidic functions. Some of the sugars were detected in nature for the O-specific polysaccharides of the immunotypes 2, 3, 4, 5 and 6 are identical to those of the polysaccharides of the 011; 0(2a)2c; 01; 010a, 10b and 07a, 7d Lányi-Bergan serological subgroups respectively, whereas no analogues have been found for the immunotypes 1 and 7. Some cross-reactions between the LPS of different immunotypes were observed in passive haemagglutination tests; the results of inhibition of passive haemagglutination and agar gel immunoprecipitation point, however, to a specificity of the LPS. Many of the LPS of the seven Pseudomonas aeruginosa immunotypes manifest rather a high cross-protective activity in active immunization tests in mice. The nature of the cross-protective activity of the LPS is discussed. |
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| Keywords: | Pseudomonas aeruginosa Lipopolysaccharides |
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