F-plasmid gene srnB+ complements the function of the S gene of phage lambda |
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Authors: | Yoshinari Ohnishi Tsuneko Ono |
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Institution: | Department of Bacteriology, School of Medicine, The University of Tokushima, Tokushima 770, Japan |
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Abstract: | Abstract The srnB + gene located on the F plasmid was assayed for its capacity to facilitate the release from infected cells of phage λ lacking the usual lytic activity. The srnB + plasmid pOY54, carrying the 1.4–2.5F fragment in the Eco RI- Bam HI fragment of pBR322, induced bacteriolysis and the release of progeny phage of the λcI 857 susS 7 lysogen in the presence of rifampin at 42°C. An srnB 1 mutant plasmid, pOY541, did not promote bacteriolysis. These results suggest that the srnB + gene of the F plasmid complements the function of the λ S gene in the nonpermissive host strain. |
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Keywords: | F-plasmid srnB gene bacteriolysis rifampin lambda S gene |
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