Rapid distinction between Leptonema and Leptospira by PCR amplification of 16S-23S ribosomal DNA spacer |
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Authors: | Tony HS Woo Lee D Smythe Meegan L Symonds Michelle A Noms Michael F Dohntb BKC Patela |
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Institution: | The Department of Molecular Life Science, Tokai University School of Medicine, Shimokasuya 143, Isehara 259-11, Japan |
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Abstract: | Abstract The protein-D2 porin of Pseudomonas aeruginosa is lacking in carbapenem or fluoroquinolone-resistant strains and hence was thought to facilitate the diffusion of these antibiotics. We examined the effect of several antibiotics on the single channel conductivity of protein-D2 in planar lipid bilayers and found that fluoroquinolones and carbapenems at concentrations of around 1 mM caused closure of the protein-D2 channel. Tetracycline, ampicillin, piperacillin, and latamoxef did not exert any detectable effect on the protein-D2 channel activity. |
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Keywords: | Outer membrane Porin Antibiotic Permeability Planar lipid bilayer Pseudomonas aeruginosa |
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