Purification of a biologically active recombinant glyceraldehyde 3-phosphate dehydrogenase from Candida albicans |
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Authors: | Eva Villamó na,Daniel Gozalboa,José P Martí neza,M.Luisa Gila |
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Affiliation: | Departamento de Microbiología y Ecología, Facultad de Farmacia, Universitat de València, Avda. Vicent Andrés Estellés, s/n, 46100 Burjassot, Valencia, Spain. |
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Abstract: | We report here the purification of a functionally active recombinant glyceraldehyde 3-phosphate dehydrogenase (GAPDH) from Candida albicans. The GAPDH protein encoded by the TDH1 gene was obtained as a glutathione S-transferase fusion protein by expression in the vector pGEX-4T-3, and purified by affinity chromatography and thrombin digestion. The purified protein displays GAPDH enzymatic activity (42 micromol NADH min(-1) mg(-1)) as well as the laminin and fibronectin binding activities previously described. In addition, the recombinant GAPDH is covalently modified by NAD linkage; this modification is stimulated by nitric oxide and probably involves a sulfhydryl group (cysteine) residue since it is inhibited by Hg(2+) and cysteine. |
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Keywords: | Candida albicans Glyceraldehyde 3-phosphate dehydrogenase TDH1 gene Recombinant protein |
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