|
|||||
|
|
Engineering the luxCDABE genes from Photorhabdus luminescens to provide a bioluminescent reporter for constitutive and promoter probe plasmids and mini-Tn5 constructs |
|
| Authors: | Michael K Winson Simon Swift Philip J Hill Catriona M Sims Gottfried Griesmayr Barrie W Bycroft Paul Williams Gordon SAB Stewart |
| Institution: | Food Microbiology Section, School of Biological Sciences, Division of Food Sciences, Sutton Bonington Campus, University of Nottingham, Nottingham, Leics. LE12 5RD, UK;School of Pharmaceutical Sciences, University Park, University of Nottingham, Nottingham NG7 5RD, UK;Anthos Labtec Instruments GmbH, Jakob-Haringerstrasse 8, A-5022 Salzburg, Austria |
| Abstract: | The luxCDABE operon of Photorhabdus luminescens has been cloned and engineered as an easily mobilisable cassette flanked by sites for commonly used restriction enzymes. Constitutive and promoter probe plasmids utilising the P. luminescens luxCDABE have been constructed using a number of compatible replicons and antibiotic markers. Complementary to these plasmids, a range of promoterless and constitutive luxCDABE mini-Tn5 derivatives has been constructed. The potential of coupling mini-Tn5 luxCDABE promoter probe transposons with automated luminometry and photometry to screen for mutants that exhibit growth phase variation in gene expression is demonstrated. |
| Keywords: | luxCDABE Mini-Tn5 Promoter probe Luminometry Photometry Quorum sensing |
| 本文献已被 ScienceDirect 等数据库收录！ | |