Effect of lipoteichoic acid on the uptake of Streptococcus pyogenes by HEp-2 cells |
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Authors: | Sela S Marouni M J Perry R Barzilai A |
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Institution: | Department of Human Microbiology, Sackler school of Medicine, Tel-Aviv University, Ramat Aviv, Israel. shlomos@post.tau.ac.il |
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Abstract: | Lipoteichoic acid (LTA) is thought to play a role in the interactions between Streptococcus pyogenes and host cells. We have examined the effect of exogenous LTA on the adherence and entry of S. pyogenes JRS4 strain into HEp-2 epithelial cells. LTA markedly inhibited bacterial entry in a concentration-dependent manner, up to 250 microg ml(-1). In contrast, LTA had only a slight inhibitory effect on adherence. LTA also inhibited the entry but not adherence of Salmonella typhimurium strain into HEp-2 cells. Binding experiments showed a dose-dependent binding of LTA to cells up to 10 microg ml(-1). Confocal laser microscopy imaging and analysis revealed that LTA was internalized by the epithelial cells and colocalized with F-actin. These results might imply that, following binding, exogenous LTA enters HEp-2 cells and exerts a cytotoxic effect that interferes with bacterial internalization. A possible target for LTA activity might be the actin cytoskeleton, which is known to be essential for bacterial uptake. |
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Keywords: | Group A streptococcus Lipoteichoic acid Internalization Adherence Streptococcus pyogenes |
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