Construction and physiological analysis of a Xanthomonas oryzae pv. oryzae recA mutant |
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| Authors: | Skorn Mongkolsuk Siritida Rabibhadana Rojana Sukchavalit Gary Vaughn |
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| Institution: | Laboratory of Biotechnology, Chulabhorn Research Institute, Lak Si, Bangkok 10210, Thailand;Department of Biotechnology, Faculty of Science, Mahidol University, Rama 6 Rd, Bangkok, Thailand |
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| Abstract: | A Xoo recA insertion inactivation mutant was constructed. The mutant, lacking RecA, showed increased sensitivity towards mutagen killing. This phenotype could be complemented by a cloned, functional recA. Unlike other bacteria, both the recA mutant and the parental strain had similar level of resistance to H2O2 killing and peroxide-induced mutagenesis. |
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| Keywords: | recA mutant Mutagen and peroxide sensitivity Mutagenesis |
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