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Purification and characterization of a neutral endoxylanase from Aspergillus nidulans |
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| Authors: | María Teresa Fernández-Espinar Francisco Piñaga Pascual Sanz Daniel Ramón Salvador Vallés |
| Institution: | Unidad de Bioingeniería, Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Jaime Roig 11, 46010 Valencia, Spain; Unidad de Cereales, Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Jaime Riog 11, 46010 Valencia, Spain |
| Abstract: | Abstract A neutral endoxylanase from a culture filtrate of Aspergillus nidulans grown on oat spelt xylan was purified to apparent homogeneity. The purified enzyme showed a single band on SDS-PAGE with a molecular mass of 22,000 and had an isoelectric point of 6.4. The enzyme was a non-debranching endoxylanase highly specific for xylans and completely free from cellulolytic activity. The xylanase showed an optimum activity at pH 5.5 and 62°C and had a K m of 4.2 mg oat spelt xylan per ml and a V max of 710 μmol min |
| Keywords: | Aspergillus nidulans Xylanase Purification |