Isolation and characterization of two distinct membrane fractions from the Gram-positive nucleotide producer Brevibacterium ammoniagenes ATCC 6872 |
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| Authors: | C Seehaus H Siede G Auling |
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| Institution: | Institut für Mikrobiologie der Universität Hannover, Hannover, F.R.G.;Max Planck Institut für Experimentelle Endokrinologie, Hannover, F.R.G. |
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| Abstract: | Abstract Bouyant density gradient centifugation of 'crude membranes' of the coryneform bacterium Brevibacterium ammoniagenes yielded two distinct membrane fractions which differed significantly in equilibrium densities (1.15 and 1.18 g/cm3), NADH dehydrogenase activity (0.29 and 0.09 μmol·min?1·mg?1), protein composition and association of ribosomes. As determined by one dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) proteins unique to either the free membrane (FM) fraction or the denser ribosome-containing complexed membrane (CM) fraction were identified. |
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| Keywords: | Brevibacterium ammoniagenes Protein secretion Complexed membrane |
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