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Capsular polysaccharide expressed by Pasteurella piscicida grown in vitro |
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| Authors: | Ramón Bonet Beatriz Magariños Jesús L Romalde Maria Dolores Simon-Pujol Alicia E Toranzo Francisco Congregado |
| Institution: | Departament de Microbiologia i Parasitologia Sanitàries, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII s/n., 08028 Barcelona, Spain; Departamento de Microbiología y Parasitología, Facultad de Biología, Universidad de Santiago de Compostela, 15706, Santiago de Compostela, Spain |
| Abstract: | Abstract Pasteurella piscicida grown in a glucose-rich medium produces a capsule that can be see under light and electron microscopy. The capsular polysaccharide was purified and characterized by chemical and HPLC analysis. The polymer has the composition glucose/mannose/ N -acetylgalactosamine/galacturonic acid/acetic acid in the molar ratios of approximately 2.5:1.3:0.5:0.4:2.5. The polysaccharide was immunogenic in rabbits and did not cross-react with antibodies against the O-antigen lipopolysaccharide. |
| Keywords: | Pasteurella piscicida Capsule Polysaccharide |