Characterization of eukaryotic-like kinase activity in Escherichia coli using the gene-protein database |
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Authors: | Scan T Sweeney Primrose Freestone Vic Norris |
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Institution: | Department of Microbiology and Immunology, Medical Sciences Building, University of Leicester, Leicester LE1 9HN, UK |
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Abstract: | Abstract The gene-protein database was used to obtain the two-dimensional polyacrylamide gel coordinates of proteins phosphorylated in extracts of Escherichia coli including those phosphorylated by eukaryotic-like kinase activities. These suggest that the phosphoproteins correspond to, or co-migrate with, the product of an open reading frame at 1.3 min (Orf80), Enzyme 1 of the phosphoenolpyruvate-dependent phosphotransferase system (Ptsl), the tRNA synthetase for histidine (HisS), and proteins involved in the response to carbon starvation and quinone treatment. |
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Keywords: | Gene-protein index Escherichia coli Protein kinase Calcium |
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