Purification and characterization of Vibrio cholerae O139 fimbriae |
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| Authors: | Tetsu Yamashiro Noboru Nakasone Yasuko Honma M John Albert Masaaki Iwanaga |
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| Institution: | Department of Bacteriology, University of the Ryukyus, School of Medicine, 207 Uehara, Okinawa 903-01, Japan; Research Institute of Comprehensive Medicine, University of the Ryukyus, School of Medicine, 207 Uehara, Nishihara, Okinawa 903-01, Japan; International Centre for Diarrheal Disease Research, GPO Box 128, Dhaka 1000, Bangladesh |
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| Abstract: | Abstract A Vibrio cholerae O139 (strain Al-1841) isolated from a patient with a cholera-like disease in Bangladesh predominantly produced new curved, wavy fimbriae (Al-1841 fimbriae) and small numbers of previously reported V. cholerae non-O1 S7-like pili. The former was purified and characterized. The molecular mass of the Al-1841 fimbrial subunit was less than 2.5 kDa, and it was immunologically different from that of V. cholerae non-O1 S7 pili. This novel fimbrial antigen was detected in all 182 Gram-negative strains from five genera tested but was absent from the Gram-positive bacteria tested. The purified Al-1841 fimbriae did not agglutinate human or rabbit erythrocytes. |
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| Keywords: | Vibrio cholerae O139 Fimbriae Immunoblotting |
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