Cloning of a haemolysin from Citrobacter freundii and its expression in phylogenetically related bacteria |
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| Authors: | Ali Al-Zaag JM Pemberton |
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| Institution: | Department of Microbiology, University of Queensland, St. Lucia, Q. 4067, Australia |
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| Abstract: | Abstract The determinants for a haemolysin from an extraintestinal isolate of Citrobacter freundii have been cloned and expressed in both Escherichia coli K12 and phylogenetically related bacteria. Compared with E. coli , where the haemolytic determinants are encoded in 7.5 kb, the hemolysin determinants of C. freundii are located on a 2.5-kb Hin dIII fragment in the recombinant plasmid PJP71. Chicken embryo tests indicate that this haemolysin does contribute to the pathogenicity of C. freundii . |
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| Keywords: | Citrobacter freundii haemolysin gene cloning expression |
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