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Multiple xylanases of Cellulomona fimi are encoded by distinct genes |
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| Authors: | Jonathan H Clarke Judith I Laurie Harry J Gilbert Geoffrey P Hazlewood |
| Institution: | Department of Biochemistry, AFRC Institute of Animal Physiology and Genetics Research, Cambridge, U.K. |
| Abstract: | Cellulomonas fimi genomic DNA encoding xylanase activity has been cloned and expressed in Escherichia coli. As judged by DNA hybridization and restriction analysis, twelve xylanase-positive clones carried a minimum of four different xylanase (xyn) genes. The encoded enzymes were devoid of cellulase activity but three of the four bound to Avicel. |
| Keywords: | ColE1 SOS induction Inducible mutagenesis Escherichia coli Salmonella typhimurium Bacillus subtilis |