多发性骨髓瘤患者外周血淋巴细胞亚群分析及与预后因子的相关性

目的通过对多发性骨髓瘤（MM）患者外周血淋巴细胞亚群的检测以评价MM患者机体的免疫功能状态。方法采用流式细胞术检测36例MM患者和25例健康志愿者外周血T、B淋巴细胞、NK细胞及CD4＋CD25＋T细胞的表达。结果与正常对照组相比,MM患者外周血的CD4、CD19细胞的表达显著下调,CD8细胞的表达显著上调,CD4/CD8比值则显著降低（P〈0.05或〈0.01）;MM患者外周血的CD4＋CD25＋T细胞占CD3＋T细胞的比例明显增高（P〈0.01）,且与血清中的β2-MG浓度成正相关（γ=0.56,P〈0.05）。结论 MM患者体内存在淋巴细胞亚群的异常表达、CD4＋CD25＋Treg细胞的异常扩增,可能是MM患者体内广泛存在免疫缺陷的一个主要原因。

Analysis of T Lymphocyte Subsets in Peripheral Blood of Patients with Multiple Myeloma and its Correlation with Clinical Prognostic Factors

Objective To evaluate immune function of patients with MM by detect the changes of the lymphocyte subsets in peripheral blood of patients with MM.Methods T lymphocyte subsets,B cells,NK cells and CD4＋CD25＋T cells in peripheral blood of 36 patients with MM and 25 healthy donors were measured by flow cytometry.Results Compared with the control group,the percentage of CD4,CD19,CD4/CD8 were reduced and the percentage of the CD8 was elevated in patients with MM.CD4＋CD25＋T cells/CD3＋T cells ratio was increased in patients with MM than that in controls,and was positively correlated with the level of β2-MG（γ=0.56,P0.05）.Conclusion MM Patients have a disorder of T,B lymphocyte subsets and have an abnormal amplification of CD4＋CD25＋Treg cells,which may be an important mechanism of MM immune deficiency.