Changes in testicular lactate dehydrogenases of the rat (Rattus norvegicus) during growth and development |
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| Authors: | A F Skidmore T J Beebee |
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| Institution: | Department of Biochemistry, University of Sussex, Falmer, Brighton, UK. |
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| Abstract: | 1. We have quantified lactate dehydrogenase (LDH) A, B and C enzyme levels during the first twelve weeks of life in rat testes. 2. Proteins were electrophoresed under non-denaturing conditions, isoenzymes identified by activity staining and quantified by densitometry scanning. 3. LDH A declined 2-3-fold during testes growth, changing from the most to the least abundant isoenzyme form. LDH B remained fairly constant, and LDH C increased from undetectable levels at birth to become the most abundant of the three polypeptides from week 3 onwards. |
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