Survival and development in culture of dissociated parasympathetic neurons from ciliary ganglia |
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| Authors: | Stephen L Helfand Gary A Smith Norman K Wessells |
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| Institution: | Department of Biological Sciences, Stanford University, Stanford, California 94305 USA |
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| Abstract: | Parasympathetic neurons from avian embryonic ciliary ganglia survive in low density culture when neurons are free from contact with other cells. A charged substratum, polyornithine, and a conditioned medium permit cell survival and vigorous neurite formation. The heart-conditioned medium must be present continuously and is active after dialysis. Neurites elongate rapidly, branch extensively, and follow patterns of charged substratum provided in the culture dish. |
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