Incipient forebrain boundaries traced by differential gene expression and fate mapping in the chick neural plate |
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Authors: | Luisa Sánchez-Arrones Lucía Rodríguez-Gallardo |
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Institution: | a Department of Human Anatomy and Psychobiology, University of Murcia, School of Medicine, Murcia, E30071, Spain b Department of Morphological Sciences and Cell Biology, University of Extremadura, School of Biology, Badajoz, E06071, Spain |
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Abstract: | We correlated available fate maps for the avian neural plate at stages HH4 and HH8 with the progress of local molecular specification, aiming to determine when the molecular specification maps of the primary longitudinal and transversal domains of the anterior forebrain agree with the fate mapped data. To this end, we examined selected gene expression patterns as they normally evolved in whole mounts and sections between HH4 and HH8 (or HH10/11 in some cases), performed novel fate-mapping experiments within the anterior forebrain at HH4 and examined the results at HH8, and correlated grafts with expression of selected gene markers. The data provided new details to the HH4 fate map, and disclosed some genes (e.g., Six3 and Ganf) whose expression domains initially are very extensive and subsequently retract rostralwards. Apart from anteroposterior dynamics, some genes soon became downregulated at the prospective forebrain floor plate, or allowed to identify an early roof plate domain (dorsoventral pattern). Peculiarities of the telencephalon (initial specification and differentiation of pallium versus subpallium) are contemplated. The basic anterior forebrain subdivisions seem to acquire correlated specification and fate mapping patterns around stage HH8. |
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Keywords: | ABB alar-basal boundary acp anterior commissure prospective aip anterior intestinal portal anb anterior-neural boundary ANR anterior neural ridge AP anterior-posterior dimension ap alar plate bp basal plate Di diencephalon DMB diencephalic-mesencephalic boundary dt digestive tube DV dorsoventral dimension ec ectoderm ef eye field ep endodermal plate fp floor plate Hb-Em Habenulo-eminential domain HN Hensen's node hp head process hypoth hypothalamus ist isthmus lp lateral plate mfp median floor plate Mes mesencephalon N notochord nc neural crest nf neural fold ne neuroectoderm nne non-neural ectoderm ov optic vesicle Pall pallium pfp paramedian floor plate POA preoptic area pp prechordal plate Rh Rhombencephalon rp roof plate SC spinal cord SP secondary prosencephalon SPall subpallium Tel telencephalon |
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