Eya1 and Six1 promote neurogenesis in the cranial placodes in a SoxB1-dependent fashion |
| |
| Authors: | Schlosser Gerhard Awtry Tammy Brugmann Samantha A Jensen Eric D Neilson Karen Ruan Gui Stammler Angelika Voelker Doris Yan Bo Zhang Chi Klymkowsky Michael W Moody Sally A |
| |
| Institution: | a Brain Research Institute, University of Bremen, FB2, PO Box 330440, 28334 Bremen, Germany;b Department of Anatomy and Regenerative Biology, Institute for Biomedical Sciences, The George Washington University, Washington, DC20037, USA;c Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309-0347, USA;d Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, 600 University Ave, Toronto, Ontario, Canada M5G 1X5 |
| |
| Abstract: | Genes of the Eya family and of the Six1/2 subfamily are expressed throughout development of vertebrate cranial placodes and are required for their differentiation into ganglia and sense organs. How they regulate placodal neurogenesis, however, remains unclear. Through loss of function studies in Xenopus we show that Eya1 and Six1 are required for neuronal differentiation in all neurogenic placodes. The effects of overexpression of Eya1 or Six1 are dose dependent. At higher levels, Eya1 and Six1 expand the expression of SoxB1 genes (Sox2, Sox3), maintain cells in a proliferative state and block expression of neuronal determination and differentiation genes. At lower levels, Eya1 and Six1 promote neuronal differentiation, acting downstream of and/or parallel to Ngnr1. Our findings suggest that Eya1 and Six1 are required for both the regulation of placodal neuronal progenitor proliferation, through their effects on SoxB1 expression, and subsequent neuronal differentiation. |
| |
| Keywords: | Xenopus NeuroD Neurogenin Otic placode Epibranchial placodes Trigeminal placode Olfactory placode Lateral line placodes p27Xic1 Cell cycle |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
