Optimization of erythritol production by Candida magnoliae in fed-batch culture |
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Authors: | Y-W Ryu C Y Park J B Park S-Y Kim J-H Seo |
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Institution: | (1) Department of Food Science and Technology, Research Center for New Biomaterials in Agriculture, Seoul National University, Suwon, 441-744, South Korea, KR;(2) Division of Molecular Science and Technology, Ajou University, Suwon, 442-749, South Korea, KR |
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Abstract: | A two-stage fed-batch process was designed to enhance erythritol productivity by the mutant strain of Candida magnoliae. The first stage (or growth stage) was performed in the fed-batch mode where the growth medium was fed when the pH of the
culture broth dropped below 4.5. The second stage (or production stage) was started with addition of glucose powder into the
culture broth when the cell mass reached about 75 g dry cell weight l−1. When the initial glucose concentration was adjusted to 400 g l−1 in the production stage, 2.8 g l−1 h−1 of overall erythritol productivity and 41% of erythritol conversion yield were achieved, which represented a fivefold increase
in erythritol productivity compared with the simple batch fermentation process. A high glucose concentration in the production
phase resulted in formation of organic acids including citrate and butyrate. An increase in dissolved oxygen level caused
formation of gluconic acid instead of citric acid. Journal of Industrial Microbiology & Biotechnology (2000) 25, 100–103.
Received 25 February 2000/ Accepted in revised form 08 June 2000 |
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Keywords: | : Candida magnoliae erythritol fed-batch culture |
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