Optimization of phytase production by solid substrate fermentation |
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| Authors: | B?Bogar G?Szakacs Email author" target="_blank">J?C?LindenEmail author A?Pandey R?P?Tengerdy |
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| Institution: | (1) Department of Agricultural Chemical Technology, Technical University of Budapest, Gellert ter 4, 1111, Budapest, Hungary,;(2) Department of Microbiology, Colorado State University, CO 80523-1677, Ft. Collins, USA,;(3) Biotechnology Division, Regional Research Laboratory, CSIR, 695 019, Trivandrum, India, |
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| Abstract: | The production of phytase by three feed-grade filamentous fungi (Aspergillus ficuum NRRL 3135, Mucor racemosus NRRL 1994 and Rhizopus oligosporus NRRL 5905) on four commonly used natural feed ingredients (canola meal, cracked corn, soybean meal, wheat bran) was studied
in solid substrate fermentation (SSF). A. ficuum NRRL 3135 had the highest yield 15 IU phytase activity/g dry matter (DM)] on wheat bran. By optimizing the supplementation
of wheat bran with starch and (NH4)2SO4, phytase production increased to 25 IU/g DM. Optimization was carried out by Plackett-Burman and central composite experimental
designs. Using optimized medium, phytase, phosphatase, alpha-amylase and xylanase production by A. ficuum NRRL 3135 was studied in Erlenmeyer flask and tray SSF. By scaling up SSF from flasks to stationary trays, activities of
20 IU phytase activity/g DM were reproducibly obtained.
Electronic Publication |
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| Keywords: | Phytase Solid substrate fermentation Aspergillus ficuum Optimization Statistical experimental design |
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