High cell density cultivation of <Emphasis Type="Italic">Escherichia coli</Emphasis> with surface anchored transglucosidase for use as whole-cell biocatalyst for α-arbutin synthesis |
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Authors: | Po-Hung Wu Giridhar R Nair I-Ming Chu Wen-Teng Wu |
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Institution: | (1) Department of Chemical Engineering, National Tsing Hua University, 101, Section 2, Kuang-Fu Road, Hsinchu, 30013, Taiwan;(2) Department of Engineering, The University of Waikato, Hamilton, 3240, New Zealand;(3) Department of Chemical Engineering, National Cheng Kung University, No.1, Ta-Hsueh Road, Tainan, 701, Taiwan |
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Abstract: | A fed-batch culture strategy for the production of recombinant Escherichia coli cells anchoring surface-displayed transglucosidase for use as a whole-cell biocatalyst for α-arbutin synthesis was developed.
Lactose was used as an inducer of the recombinant protein. In fed-batch cultures, dissolved oxygen was used as the feed indicator
for glucose, thus accumulation of glucose and acetate that affected the cell growth and recombinant protein production was
avoided. Fed-batch fermentation with lactose induction yielded a biomass of 18 g/L, and the cells possessed very high transglucosylation
activity. In the synthesis of α-arbutin by hydroquinone glucosylation, the whole-cell biocatalysts showed a specific activity
of 501 nkat/g cell and produced 21 g/L of arbutin, which corresponded to 76% molar conversion. A sixfold increased productivity
of whole cell biocatalysts was obtained in the fed-batch culture with lactose induction, as compared to batch culture induced
by IPTG. |
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Keywords: | Escherichia coli Surface-display Transglucosidase Induction Fed-batch |
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