Succinic acid production from sugarcane bagasse hemicellulose hydrolysate by <Emphasis Type="Italic">Actinobacillus succinogenes</Emphasis> |
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Authors: | Elcio Ribeiro Borges Jr" target="_blank">Nei PereiraJr |
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Institution: | (1) Bioprocess Development Laboratories, School of Chemistry, Technology Center, Federal University of Rio de Janeiro, Rio de Janeiro, 21949-900, Brazil; |
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Abstract: | Succinic acid, a four-carbon diacid, has been the focus of many research projects aimed at developing more economically viable
methods of fermenting sugar-containing natural materials. Succinic acid fermentation processes also consume CO2, thereby potentially contributing to reductions in CO2 emissions. Succinic acid could also become a commodity used as an intermediate in the chemical synthesis and manufacture
of synthetic resins and biodegradable polymers. Much attention has been given recently to the use of microorganisms to produce
succinic acid as an alternative to chemical synthesis. We have attempted to maximize succinic acid production by Actinobacillus succinogenes using an experimental design methodology for optimizing the concentrations of the medium components. The first experiment
consisted of a 24−1 fractional factorial design, and the second entailed a Central Composite Rotational Design so as to achieve optimal conditions.
The optimal concentrations of nutrients predicted by the model were: NaHCO3, 10.0 g l−1; MgSO4, 3.0 g l−1; yeast extract, 2.0 g l−1; KH2PO4. 5.0 g l−1; these were experimentally validated. Under the best conversion conditions, as determined by statistical analysis, the production
of succinic acid was carried out in an instrumented bioreactor using sugarcane bagasse hemicellulose hydrolysate, yielding
a concentration of 22.5 g l−1. |
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