Effects of nutrient dosing on subsurface methanotrophic populations and trichloroethylene degradation |
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Authors: | S M Pfiffner A V Palumbo T J Phelps T C Hazen |
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Institution: | (1) Environmental Sciences Division, Oak Ridge National Laboratory, PO Box 2008, Oak Ridge, TN 37831-6036, USA, US;(2) Westinghouse Savannah River Technology Center, Aiken, SC, USA, US |
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Abstract: | In in situ bioremediation demonstration at the Savannah River Site in Aiken, South Carolina, trichloroethylene-degrading microorganisms
were stimulated by delivering nutrients to the TCE-contaminated subsurface via horizontal injection wells. Microbial and
chemical monitoring of groundwater from 12 vertical wells was used to examine the effects of methane and nutrient (nitrogen
and phosphorus) dosing on the methanotrophic populations and on the potential of the subsurface microbial communities to
degrade TCE. Densities of methanotrophs increased 3–5 orders of magnitude during the methane- and nutrient-injection phases;
this increase coincided with the higher methane levels observed in the monitoring wells. TCE degradation capacity, although
not directly tied to methane concentration, responded to the methane injection, and responded more dramatically to the multiple-nutrient
injection. These results support the crucial role of methane, nitrogen, and phosphorus as amended nutrients in TCE bioremediation.
The enhancing effects of nutrient dosing on microbial abundance and degradative potentials, coupled with increased chloride
concentrations, provided multiple lines of evidence substantiating the effectiveness of this integrated in situ bioremediation process.
Received 13 November 1995/ Accepted in revised form 12 September 1996 |
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Keywords: | : in situ bioremediation subsurface methanotrophs TCE nutrient availability |
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