Pectinase production by <Emphasis Type="Italic">Aspergillus giganteus</Emphasis> in solid-state fermentation: optimization,scale-up,biochemical characterization and its application in olive-oil extraction |
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Authors: | Gastón E Ortiz María C Ponce-Mora Diego G Noseda Gabriela Cazabat Celina Saravalli María C López Guillermo P Gil Martín Blasco Edgardo O Albertó |
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Institution: | 1.Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECH),Universidad Nacional de San Martín (UNSAM)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET),San Martín,Argentina;2.Centro de Investigación y Desarrollo en Tecnologías de Industrialización de Alimentos,Instituto Nacional de Tecnología Industrial,San Martín,Argentina;3.Centro de Investigación y Desarrollo en Biotecnología Industrial,Instituto Nacional de Tecnología Industrial,San Martín,Argentina |
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Abstract: | The application of pectinases in industrial olive-oil processes is restricted by its production cost. Consequently, new fungal strains able to produce higher pectinase titers are required. The aim of this work was to study the capability of Aspergillus giganteus NRRL10 to produce pectinolytic enzymes by SSF and evaluate the application of these in olive-oil extraction. A. giganteus was selected among 12 strains on the basis of high pectinolytic activity and stability. A mixture composed by wheat bran, orange, and lemon peels was selected as the best substrate for enzyme production. Statistical analyses of the experimental design indicated that pH, temperature, and CaCl2 are the main factors that affect the production. Subsequently, different aeration flows were tested in a tray reactor; the highest activity was achieved at 20 L min?1 per kilogram of dry substrate (kgds). Finally, the pectinolytic enzymes from A. giganteus improved the oil yield and rheological characteristics without affecting oil chemical properties. |
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