水稻Ac×Ds后代基因组DNA中Ds侧翼序列的扩增及其Ds插入分析

采用TAIL-PCR技术从经鉴定含Ac/Ds双元件的材料中扩增Ds侧翼序列并测序, 对水稻Ac×Ds后代基因组DNA进行Ac和Ds插入的PCR分析。利用NCBI的BLAST软件, 以Ds侧翼序列为待查询序列进行GenBank在线搜索比对, 获得Ds插入相关基因的染色体定位和功能注释等信息。对扩增的93个有效Ds侧翼序列进行分析, 结果显示, 有21个水稻杂交后代中Ds插入于基因编码区, 其余72个插入在基因间序列, 其中12个插入在特定基因的上游3 kb以内的间隔区。本研究强调了提高Ds侧翼序列扩增和Ac/Ds植株筛选效率的技术关键。

Amplification of Ds Flanking Sequences from Rice Genomic DNA of Hybrids of Ac × Ds Lines and the Analysis of Ds Insertions

Ac and Ds insertions among the genomic DNAs of hybrids of Ac x Ds lines were screened by PCR. The genomic DNAs, which were proved to harbour both Ac and Ds, were used as templates in TAIL-PCR to clone the Ds flanking sequences. The cloned specific fragments were sequenced, and the sequenced Ds flanking sequences were used as query sequences to perform on-line sequence comparing analysis against GenBank by employing BLAST program of NCBI. The information about the chromosome location of Ds-inserted genes, or genes immediately downstream of the inserted sites, and their functional innotations were achieved. Based on the analysis from the cloned 93 Ds-flanking sequences, it was found that 21 hybrid plants had Ds insertions in genic regions, whereas the remaining 72 samples's intergenic regions were inserted by Ds element. Moreover, among the 72 regions, 12 were inserted immediately upstream (within 3 kb) of specific genes. Also, the strategies to improve the performance in cloning the Ds flanking sequences and in screening the Ac/Ds lines were emphasized.