4个HLA等位基因在5个民族中分布的多样性研究

应用ARMS-SSP结合的方法调查与AIDS相关的HLA等位基因，即HLA-A*02，B*35，B*27，B*57四个基因在五个民族群体（云南汉族、彝族、傣族、新疆维吾尔族、广西壮族）中的分布情况，并进行了相应的遗传学分析。结果显示，HLA-A*02在壮族和汉族中检出的阳性样本频率非常高，在另外几个民族中的分布频率偏低；B*35基因在各民族中分布差异不大;B*27在傣族中的分布频率较高，汉族、彝族和壮族中分布的频率差异不大；B*57则在各民族中的分布差异不大；保护性的等位基因即A*02，B*27，B*57的基因型频率在5个民族中有极显著的差异。此课题增进了对我国部分AIDS流行地区AIDS相关HLA等位基因型遗传背景的了解，是对HLA与AIDS相关性研究资料的有益补充。

Polymorphism Research on the Distribution of Four HLA Alleles in Five Chinese Populations

ARMS-SSP PCR was applied to survey the distribution of four AIDS associated HLA alleles, HLA-A*02, B*35, B*27, and B*57 in five Chinese nationalities (Yunnan han, yi, dai, Xinjiang uygur, and Guangxi zhuang).The results showed that HLA-A*02 allele occured very frequently at higher frequencies in han and zhuang than in the other three nationalities; B*35 did not show great variation in it's distribution in five population groups; B*27 had a distinctively high frequency in dai, but normal in han, yi and zhuang; B*57 allele did not vary greatly in these nationalities, too. A*02, B*27 and B*57 are three protective alleles. As a whole, their frequencies in the five nationalities were significantly different. This study could be a complement of the HLA and AIDS relative studies, and enhanced the understanding of the genetic background of some AIDS epidemic regions in China.