| 一种简便、快速的大肠杆菌质粒转化方法 |
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| 引用本文： | 郭培懿,陈向东,谢志雄,沈萍GUO Pei-yi,CHEN Xiang-dong,XIE Zhi-xiong,SHEN Ping.一种简便、快速的大肠杆菌质粒转化方法[J].遗传,1999,21(4):48-51. |
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| 作者姓名： | 郭培懿 陈向东 谢志雄 沈萍GUO Pei-yi CHEN Xiang-dong XIE Zhi-xiong SHEN Ping |
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| 作者单位： | 武汉大学生命科学学院,湖北武汉 430072
College of Life Sciences,Wuhan University,Wuhan 430072,China |
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| 摘 要： | 将受体菌与质粒DNA混匀直接在Ca2+离子选择平板上进行转化和筛选，其转化过程仅需2 min左右，并能得到105以上的转化效率, 可满足一般克隆工作的需要。
Abstract：After mixing the recipient cells and plasmids DNA, directly spread the mixture on selective media containing Ca2+. The whole process of transformation just needs 2 min or so, and could acquire the transformation efficiency of more than 105, which is enough to common gene cloning.
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| 关 键 词： | 转化 大肠杆菌 Transformation Escherichia coli Ca2+选择平板 Key words 大麦黄矮病抗性 细胞遗传 易位系 附加系 杂种幼胚培养 |
| 修稿时间： | 1998-11-03 |
A Simple and Rapid Method for the Transformation of Escherichia coli by Plasmids |
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| GUO Pei-yi,CHEN Xiang-dong,XIE Zhi-xiong,SHEN Ping.A Simple and Rapid Method for the Transformation of Escherichia coli by Plasmids[J].Hereditas,1999,21(4):48-51. |
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| Authors: | GUO Pei-yi CHEN Xiang-dong XIE Zhi-xiong SHEN Ping |
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| Abstract: | After mixing the recipient cells and plasmids DNA, directly spread the mixture on selective media containing Ca 2 The whole process of transformation just needs 2 min or so, and could acquire the transformation efficiency of more than 10 5 , which is enough to common gene cloning |
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| Keywords: | Transformation Escherichia coli Ca 2 selective plateZ BSTRACTBSTRACT The atomic quantum behaviors in the system of two\|mode squeezed vacuum field interacting with a two\|level atom via two\|photon process are studied by means of qua |
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