山羊GOLA-DQB1基因外显子2多态性与免疫性状的相关分析

利用PCR-RFLP技术, 对莱芜黑山羊、鲁波山羊和波尔山羊3个山羊种群共 175 只个体的GOLA-DQB1基因外显子2进行遗传多态性研究, 并对山羊种群的血液免疫指标的效应进行了分析。结果表明: 3个山羊种群共检测到(AA、BB、CC、AB、AC、BC、DD)7种基因型, GOLA-DQB1基因外显子2的第24、151位的碱基表现出多态性。多数指标品种效应是主要效应。莱芜黑山羊中, BC基因型的淋巴细胞百分比(W-SCR)显著高于AC 、CC基因型(P<0.05), 中性球比例(W-LCR)显著低于CC基因型(P<0.05), 大型白细胞数(W-LCC)低于AC、CC基因型, 但差异不显著(P>0.05)。波尔山羊中, BC基因型的W-LCC低于AA 、AB 、BB基因型, 但差异不显著(P>0.05)。鲁波山羊中, BC 、AC基因型的W-LCC显著低于AA基因型(P<0.05)。揭示GOLA-DQB1基因与血液免疫性状有一定的相关性。

Association of polymorphisms of exon 2 of GOLA-DQB1 gene with immune traits in goats

PCR-RFLP was used to analyze the polymorphisms of exon 2 of GOLA-DQB1 gene in Laiwu black goats, Lubo goats, and Boer goats. The effects of genotypes of exon 2 of GOLA-DQB1 gene on immune traits were estimated. Seven genotypes (AA, BB, CC, AB, AC, BC, and DD) were detected by analyzing restriction maps. Polymorphic sites were detected at base position 24, 151 of exon 2 of GOLA-DQB1 gene. The results showed that the effects of breed were major effect. W-SCR in Laiwu black goats with genotype BC was significantly higher than that with genotype AC and CC (P<0.05), W-LCR in Laiwu black goats with genotype BC was significantly lower than that with genotype CC (P<0.05); W-LCC in Laiwu black goats with genotype BC was lower than that with genotype AC, CC (P>0.05). W-LCC in Boer goats with genotype BC was lower than that with genotype AA, AB, and BB (P>0.05); W-LCC in Lubo goats with genotype BC and AC was significantly lower than that with genotype AA (P<0.05). It was concluded from the results that GOLA-DQB1 was the gene affecting the immune traits. These results may be applied to marker assisted selection in disease resistance breeding of goats.