| PCR反应混合液的冻干处理及其应用 |
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| 引用本文： | 汪秀峰,李莉,皮桃花,倪大虎,张毅,向太和,杨剑波.PCR反应混合液的冻干处理及其应用[J].遗传,2002,24(2):171-173. |
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| 作者姓名： | 汪秀峰 李莉 皮桃花 倪大虎 张毅 向太和 杨剑波 |
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| 作者单位： | 安徽省农业科学院 农业部水稻遗传育种重点开放实验室，合肥 230031
Key Laboratory of Rice Genetics and Breeding for Agricultural Ministry of China,Anhui Academy of Agricultural Sciences,Hefei 230031 |
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| 基金项目： | 安徽省政府专项基金资助种子工程项目 |
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| 摘 要： | 本研究将除模板DNA以外的PCR反应混合液，经过真空浓缩冻成干粉状后，分别置于室温和4℃贮存，经过一定的贮藏时间后进行PCR反应，发现经冻干处理的样品能在较长的贮存时间内保持扩增活性，这将给实际工作带来很大的便利。
Abstract:In this paper we introduce a new storage method of PCR ingredient.PCR mixture except DNA template has been frozen to dry powder by the DNA-Plus system.This kind of powder was stored at room temperature or 4℃.PCR has been run in different period of storage.It was discovered that the samples of lyophilization could keep activity for a long time.
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| 关 键 词： | 冻干 贮存 storage lyophilization PCR扩增 Key words |
| 文章编号： | 0253-9772(2002)02-0171-03 |
| 修稿时间： | 2001年7月26日 |
Lyophilized Mixture of PCR Ingredients and Its Application |
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| WANG Xiu-feng,LI Li,PI Tao-hua,NI Da-hu,ZHANG Yi,XIANG Tai-he,YANG Jian-bo.Lyophilized Mixture of PCR Ingredients and Its Application[J].Hereditas,2002,24(2):171-173. |
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| Authors: | WANG Xiu-feng LI Li PI Tao-hua NI Da-hu ZHANG Yi XIANG Tai-he YANG Jian-bo |
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| Institution: | Key Laboratory of Rice Genetics and Breeding for Agricultural Ministry of China, Anhui Academy of Agricultural Sciences, Hefei 230031. bioinfo@mail.hf.ah.cn |
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| Abstract: | In this paper we introduce a new storage method of PCR ingredient. PCR mixture except DNA template has been frozen to dry powder by the DNA-Plus system. This kind of powder was stored at room temperature or 4 degrees. PCR has been run in different period of storage. It was discovered that the samples of lyophilization could keep activity for a long time. |
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| Keywords: | lyophilization storage PCR amplification |
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