| 兰属Cymbidium植物ISSR遗传多样性分析 |
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| 引用本文： | 吴振兴,王慧中,施农农,赵艳.兰属Cymbidium植物ISSR遗传多样性分析[J].遗传,2008,30(5):627-632. |
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| 作者姓名： | 吴振兴 王慧中 施农农 赵艳 |
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| 作者单位： | 1. 杭州师范大学生物化学与分子生物学杭州市重点实验室,杭州,310018
2. 浙江工商大学食品、生物与环境工程学院,杭州,310018 |
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| 基金项目： | 国家自然科学基金，浙江省自然科学基金，浙江省科技计划，杭州市科技科技计划项目，浙江省新世纪151人才工程项目 |
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| 摘 要： | 将简单重复序列区间扩增多态标记(inter-simple sequence repeats, ISSR)技术应用于16种兰属的遗传多样性分析, 15个引物共扩增出836条带, 其中有227条多态带, 多态百分比为27.2%。UPGMA聚类结果显示: 春兰与春剑的亲缘关系最近, 而兔耳兰与其他15种兰属植物的距离最远。ISSR聚类结果与传统分类结果基本相似, 表明该技术能在分子水平上对传统兰属分类进行必要补充。
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| 关 键 词： | 兰属植物 遗传多样性 ISSR |
| 收稿时间： | 2007-10-10 |
| 修稿时间： | 2007年10月10 |
The genetic diversity of Cymbidium by ISSR |
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| WU Zhen-Xing,WANG Hui-Zhong,SHI Nong-Nong,ZHAO Yan.The genetic diversity of Cymbidium by ISSR[J].Hereditas,2008,30(5):627-632. |
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| Authors: | WU Zhen-Xing WANG Hui-Zhong SHI Nong-Nong ZHAO Yan |
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| Institution: | Key Laboratory of Biochemistry and Molecular Biology, Hangzhou Normal University, Hangzhou 310018,China. wuzhenxing007@126.com |
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| Abstract: | ISSR was applied to detect the relationship between 16 Cymbidium species, and 836 bands were amplified with 15 primers, including 227 polymorphic bands. The polymorphic percentage is 27.2%. UPGMA results showed that the genetic distance were closest between C.goeringii (Rchb.f.) Rchb.f. and C. goeringii var. longibracteatum, and C.lancifolium Hook. was far away from the other 15 species. This result is quite similar to the traditional classification, indicating that the technique could supplement some information to traditional taxonomy in the molecular level. |
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| Keywords: | ISSR |
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