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西部地区主要猪种和野猪H-FABP基因分子标记
引用本文:庞卫军,孙世铎,李影,陈国柱,杨公社.西部地区主要猪种和野猪H-FABP基因分子标记[J].遗传,2005,27(3):351-356.
作者姓名:庞卫军  孙世铎  李影  陈国柱  杨公社
作者单位:西北农林科技大学动物科技学院,杨陵 712100
基金项目:国家自然科学基金,高等学校博士学科点专项科研项目
摘    要:利用PCR-RFLP(Hinf I、Hae Ⅲ和Msp I 3种限制性内切酶)分子标记技术,检测了杜洛克猪、长白猪、大白猪、内江猪、荣昌猪、汉江黑猪、汉中白猪、八眉猪和野猪共计265头猪H-FABP基因5′-上游区和第二内含子区的遗传变异,并利用最小二乘模型分析了H-FABP基因对猪肌内脂肪含量的遗传效应。结果表明:(1)在Hinf I-RFLP位点上,上述品种和野猪均存在多态性,其中大白猪、八眉猪、汉江黑猪、汉中白猪和野猪表现为低度多态,杜洛克、长白猪、内江猪和荣昌猪为中度多态;除汉江黑猪(P<0.05)和野猪(P<0.01)外,其他猪种基因频率和基因型频率都处于Hardy-Weinderg平衡状态(P>0.05);而在Hae Ⅲ-RFLP和Msp I-RFLP位点上,仅内江猪、荣昌猪、汉江黑猪和八眉猪为单态;(2)9种基因型对肌内脂肪(IMF)含量的影响,HH>Hh>hh,DD
关 键 词:分子标记  遗传效应  心脏脂肪酸结合蛋白基因  肌内脂肪含量  
文章编号:0253-9772(2005)03-0351-06
收稿时间:2004-04-07
修稿时间:2004年4月7日

Relationship Between Molecular Marker of Western Main Pig H-FABP Gene and IMF Content
PANG Wei-jun,SUN Shi-duo,LI Ying,CHEN Guo-Zhu,YANG Gong-she.Relationship Between Molecular Marker of Western Main Pig H-FABP Gene and IMF Content[J].Hereditas,2005,27(3):351-356.
Authors:PANG Wei-jun  SUN Shi-duo  LI Ying  CHEN Guo-Zhu  YANG Gong-she
Institution:College of Animal Science ,Northwest Sci-Tech University of Agriculture and Forestry
Abstract:By using 265 pigs from eight breeds including Duroc,Landrace,Large White,Neijiang,Rongchang,Hanjiang Black,Hanzhong White,Bamei and wild ones, the genetic variations of 5'-upstream region from and the second intron in porcine H-FABP gene were checked by PCR-RFLP molecular marker with HinfI, Hae III and MspI,and effect of H-FABP gene on IMF content was then analyzed by least square analysis.The results showed as follows:(1) 8 pig breeds and wild pig had polymorphism at Hinf I-RFLP site. In above detected breeds,large white,Bamei pig, Hanjiang Black,Hanzhong White pig breeds and wild pig presented low polymorphism while other breeds have mediate polymorphism;(2)Among the tested breeds only 4 Chinese local pig breeds had no polymorphism at the Hae III-RFLP and Msp I-RFLP sites,but Duroc,Landrace,Largewhite, Hanzhong White pig breeds and wild pig had polymorphism. Wild pig at the Hae III-RFLP , Landrace,Largewhite and wild pig at the Hae III-RFLP and Msp I-RFLP sites were low polymorphism,others were mediate polymorphism;(3) H-FABP gene increased IMF content significantly(p0.05). Genetic effect of H-FABP gene on IMF content were HH>Hh>hh,DD.
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