| 桃ACO基因反义转化桃幼胚子叶的研究 |
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| 引用本文： | 吴延军,张上隆,谢鸣,陈俊伟,蒋桂华,秦永华,秦巧平.桃ACO基因反义转化桃幼胚子叶的研究[J].遗传,2006,28(1):65-70. |
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| 作者姓名： | 吴延军 张上隆 谢鸣 陈俊伟 蒋桂华 秦永华 秦巧平 |
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| 作者单位： | （1.浙江大学农业与生物技术学院园艺系,杭州310029； 2.浙江省农业科学院园艺所,杭州 310021） |
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| 基金项目： | 浙江省宁波市科技局资助项目，浙江省农业科学院科研项目 |
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| 摘 要： | 用根癌农杆菌(Agrobacterium tumefaciens )和基因枪两种方法，以桃(Prunus persica L. Batsch)幼胚子叶为受体，转化桃ACC氧化酶（ACO）反义基因片段，经筛选培养获得了卡那霉素抗性芽。微芽嫁接培养抗性芽部分可成株。PCR、Southern 杂交和GUS基因表达等分子检测，初步表明外源反义ACO基因已经整合到桃基因组中。
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| 关 键 词： | 基因枪 转基因 根癌农杆菌 桃 反义ACO基因 |
| 文章编号： | 0253-9772(2006)01-0065-06 |
| 收稿时间： | 2004-12-14 |
| 修稿时间： | 2005-01-31 |
Genetic Transformation of Peach Immature Cotyledons with Its Antisenes ACO Gene |
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| WU Yan-Jun,ZHANG Shang-Long,XIE Ming,CHEN Jun-Wei,JIANG Gui-Hua,QIN Yong-Hua,QIN Qiao-Ping.Genetic Transformation of Peach Immature Cotyledons with Its Antisenes ACO Gene[J].Hereditas,2006,28(1):65-70. |
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| Authors: | WU Yan-Jun ZHANG Shang-Long XIE Ming CHEN Jun-Wei JIANG Gui-Hua QIN Yong-Hua QIN Qiao-Ping |
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| Institution: | (1. Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hongzhou 310029, China; 2. Institute of Horticulture, Zhejiang Academy of Agricultural Sciences Hangzhou 310021, China) |
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| Abstract: | Genetic transformation of peach immature cotyledons with its ACO antisense gene was studied by using particle bombardment method through Agrobacterium tumefaciens.Kan~r shoots and Kan~r plantlet were obtained.The plantet with ACO antisenes gene through Agrobacterium tumefaciens was obtained by micrografting technique and survived for nearly one month.The results of the PCR,PCR-southern,genomes southern hybridization analysis and GUS color reaction of some Kan~r materials showed in some degree that the peach ACO antisens gene was integrated into peach genomes. |
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| Keywords: | peach Agrobacterium tumefaciens particle bombardment genetic transformation antisense ACO |
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