不同生长时期基因调控对南阳牛生长发育的影响

以南阳牛为试验动物，利用PCR-SSCP和PCR-RFLP技术研究了南阳牛生长激素（GH）基因，IGF-I基因以及IGF-IBP3基因的遗传多态，并分析了遗传多态位点对南阳牛0月龄、6月龄、12月龄、18月龄、24月龄以及36月龄不同生长时期生长发育性状的影响。结果表明： 在6月龄～18月龄，GH基因的GH-P5位点的BB基因型对南阳牛的体长、体高显著的正效应；在生长的后期（24月龄～36月龄），IGF-IBP3-P5基因位点对南阳牛的后躯发育起主要调控作用，BB基因型个体的尻宽显著高于AA型。这表明基因的正效应不是在所有的生长周期表现，而是在特定的时期表现对南阳牛生长发育的显著影响。

The modulation and control on growth and development traits of Nanyang Cattle in different phase

This study was conducted to identify polymorphisms of the Nangyang cattle's growth hormone(GH) and insulin-like growth factor-I(IGF-I) and insulin-like growth factor-I binding protein 3(IGF-IBP3) gene by the single nucleotide polymorphisms and polymerase chain reaction-based restriction fragment length polymorphism and to study association of polymorphisms identified in these genes with growth traits of the birth,6 months,12 months,18 months,24 months and 36 months.Results from the analysis showed a significant association of the BB genotype in the promoter of GH gene(GH-P5) with higher body length and body height from 6 months to 18 months.From 24 months to 36 months,the IGF-IBP3 locus has a dominance modulation and control effect on backbody in Nanyang Cattle,and the BB genotype with higher Rump width than AA.