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延边黄牛背最长肌差异表达基因的筛选、克隆及序列分析
作者姓名:Tian WN  Zhang SF  Li XZ  Gao QS  Jin X  Yan CG
作者单位:1. 吉林农业科技学院动物科学学院,吉林,132101
2. 延边大学农学院,延吉,133002
基金项目:“十一五”国家科技支撑计划项目(2008BADB2B01)资助
摘    要:应用引物复性控制技术筛选肌内脂肪含量差异极显著的延边黄牛背最长肌组织差异表达基因,寻找与肌内脂肪沉积的相关候选基因。文章选取30头28月龄延边黄牛阉牛的背最长肌组织测定肌内脂肪含量,选取肌内脂肪含量差异极显著的最高和最低各3头组成RNA池,采用引物复性控制技术,分析了两组个体背最长肌组织差异表达基因。利用20对随机引物差异显示扩增下,共获得12条ESTs(片段大小为200~890 bp),其中8个为已知的ESTs分别与细胞骨架形成、细胞因子信号转导、蛋白质合成、能量代谢和其他功能的差异基因,4个未知的ESTs。结果表明,应用引物复性控制技术筛选得到了12个可能参与了肌内脂肪沉积调控的ESTs,为进一步筛选肌内脂肪沉积相关的基因奠定了基础。

关 键 词:延边黄牛  背最长肌  肌内脂肪  引物复性控制技术  差异表达基因

Screening, cloning and sequence analysis of the differential expression genes in Longissimus dorsi of Yanbian yellow cattle
Tian WN,Zhang SF,Li XZ,Gao QS,Jin X,Yan CG.Screening, cloning and sequence analysis of the differential expression genes in Longissimus dorsi of Yanbian yellow cattle[J].Hereditas,2011,33(11):1219-1224.
Authors:Tian Wan-Nian  Zhang Shou-Fa  Li Xiang-Zi  Gao Qing-Shan  Jin Xin  Yan Chang-Guo
Institution:TIAN Wan-Nian1,ZHANG Shou-Fa2,LI Xiang-Zi2,GAO Qing-Shan2,JIN Xin2,YAN Chang-Guo2 1.College of Animal Science,Jilin Agricultural Science and Technology College,Jilin 132101,China,2.College of Agriculture,Yanbian University,Yanji 133002
Abstract:Annealing control primer(ACP) system was applied to find candidate genes related to lipidosis in muscle of Yanbian yellow cattle by screening differentially expressed genes(DEGs) in Longissimus dorsi,which had significant difference on intramuscular fat(IMF) content.Thirty steers,aged at 28 month-bullocks were selected to measure the IMF content in L.dorsi.Two groups of bullocks(three heads per group) with the highest and the lowest contents of IMF were selected to build a RNA pool,and DEGs of two groups we...
Keywords:Yanbian yellow cattle  Longissimus dorsi  intramuscular fat  annealing control primer(ACP) system  differential expression genes(DEGs)  
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