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| 两系杂交小麦恢复系MR168抗条锈病基因遗传分析及分子标记定位 | |
| 作者姓名： | Ren Y Li SR Li J Zhou Q DU XY Li TJ Yang WY Zheng YL |
| 作者单位： | 1. 绵阳市农业科学研究院,国家小麦改良中心绵阳分中心,绵阳 621023;四川农业大学小麦研究所,成都 611130 2. 绵阳市农业科学研究院,国家小麦改良中心绵阳分中心,绵阳 621023 3. 四川省农业科学院作物研究所,成都,610066 4. 四川农业大学小麦研究所,成都,611130 |
| 基金项目： | 国家高技术研究发展计划项目(863计划)(编号:2009AA101102); 现代农业产业技术体系建设专项资金(编号:nycytx-03)资助 |
| 摘 要： | 小麦条锈病是影响杂交小麦普及推广的重要因素。文章利用基因推导法和SSR分子标记技术,研究了温光型两系杂交小麦恢复系MR168的抗条锈性遗传规律及其控制基因染色体位置。结果表明,MR168对CY29、CY31、CY32、CY33等条锈菌生理小种表现高抗至免疫;对SY95-71/MR168杂交组合的正反交F1、BC1、F2和F3群体分单株接种鉴定显示,MR168对CY32号小种的抗性受1对显性核基因控制,该抗病基因来源于春小麦品种辽春10号。利用集群分离分析法(Bulked segregant analysis,BSA)和简单重复序列(Simple sequence repeat,SSR)分子标记分析抗病亲本MR168、感病亲本SY95-71及183个F2代单株,发现了与MR168抗条锈病基因连锁的5个微卫星标记Xgwm273、Xgwm18、Xbarc187、Xwmc269、Xwmc406,并将该基因初步定位在1BS着丝粒附近,暂命名为YrMR168;构建了包含YrMR168的SSR标记遗传图谱,距离YrMR168最近的两个微卫星位点是Xgwm18和Xbarc187,遗传距离分别为1.9 cM和2.4 cM,这两个微卫星标记可用于杂交小麦抗条锈病分子标记辅助育种。 |
| 关 键 词： | 杂交小麦 恢复系 抗条锈病基因 基因推导 遗传分析 微卫星标记 |
Genetic analysis and molecular mapping of stripe rust resistance gene in a restore line of Thermo-Photo sensitive hybrid wheat MR168 |
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| Ren Y,Li SR,Li J,Zhou Q,DU XY,Li TJ,Yang WY,Zheng YL.Genetic analysis and molecular mapping of stripe rust resistance gene in a restore line of Thermo-Photo sensitive hybrid wheat MR168[J].Hereditas,2011,33(11):1263-1270. | |
| Authors: | Ren Yong Li Sheng-Rong Li Jun Zhou Qiang DU Xiao-Ying Li Tai-Jun Yang Wu-Yun Zheng You-Liang |
| Institution: | REN Yong1,3,LI Sheng-Rong1,LI Jun2,ZHOU Qiang1,DU Xiao-Ying1,LI Tai-Jun1,YANG Wu-Yun2,ZHENG You-Liang3 1.Mianyang Branch of National Wheat Improvement Center,Mianyang Academy of Agricultural Sciences,Mianyang 621023,China,2.Crop Research Institute,Sichuan Academy of Agricultural Sciences,Chengdu 610066,China 3.Triticeae Research Institute,Sichuan Agricultural University,Chengdu 611130 |
| Abstract: | Stripe rust,caused by Puccinia striiformis f.sp.tritici,is an important limiting factor to popularize hybrid wheat.The objectives of this study were to map a stripe rust resistance gene in a Chinese thermo-photo-sensitive hybrid wheat restore line MR168 using gene postulation and SSR markers.MR168 was highly resistant to 23 Pst races including CYR29,CYR31,CYR32,and CYR33.The populations F1,BC1,F2,and F3 from the cross between MR168 and SY95-71(a wheat cultivar susceptible to Pst races) were inoculated with ... |
| Keywords: | hybrid wheat restore line stripe rust resistance gene gene postulation SSR marker |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |