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藏羚羊mtDNA D-Loop区遗传多样性研究
引用本文:周慧,李迪强,张于光,易湘蓉,刘毅.藏羚羊mtDNA D-Loop区遗传多样性研究[J].遗传,2006,28(3):299-305.
作者姓名:周慧  李迪强  张于光  易湘蓉  刘毅
作者单位:1.湖南农业大学动物科学与技术学院,湖南 长沙 410128;2.中国林业科学研究院森林生态环境与保护研究所,北京 100091;3.湖南农业大学生物安全科学与技术学院,湖南 长沙 410128
基金项目:科技部社会公益研究项目,科技部专项基金
摘    要:该研究采用非损伤性DNA基因分型技术,对可可西里地区10个藏羚羊(Pantholops hodgsonii)个体的mtDNA非编码区部分片段(444~446bp)进行了序列分析,结果显示A、T%含量(61.8%)明显高于G、C%含量(38.2%),共发现10种单倍型,包括48个多态位点,其中转换位点44个、颠换位点1个、插入位点1个、缺失位点2个。单倍型间平均遗传距离为0.031,单倍型多态性(h)为1.000,核苷酸多态性(π)为2.96%。说明藏羚羊线粒体控制区存在着丰富变异,最后从藏羚羊的生态习性及地理分布两方面对这一结果进行了分析探讨。

关 键 词:遗传多样性  单倍型  线粒体D-loop区  藏羚羊  
文章编号:0253-9772(2006)03-0299-07
收稿时间:2005-02-21
修稿时间:2005年2月21日

Study on mitochondrial DNA genetic diversity of Tibetan antelope
ZHOU Hui,LI Di-Qiang,ZHANG Yu-Guang,YI Xiang-Rong,LIU Yi.Study on mitochondrial DNA genetic diversity of Tibetan antelope[J].Hereditas,2006,28(3):299-305.
Authors:ZHOU Hui  LI Di-Qiang  ZHANG Yu-Guang  YI Xiang-Rong  LIU Yi
Institution:1. College of Animal Science and Technology of Hunan Agricultural University, Changsha 410128, China; 2. Institute of Forestry Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091,China; 3. College of Biosafety Science and Technology of Hunan Agricultural University, Changsha 410128, China
Abstract:With noninvasive DNA genotyping technology,we investigated genetic diversity of Tibetan antelope by(analyzing) mitochondrial DNA variation,those samples are collected from Hoh Xil National Nature Reserve,Qinghai province,China.A total of 444~446 bp of the mitochondrial control region was sequenced form 10 individuals.The(results) showed that A%+T%(61.8%) was higher than C%+G%(38.2%) obviously.Ten haplotypes were identified in the 10 samples.The result showed 48 polymorphic sites after comparisons of the 10 haplotype samples,among them,including 44 transitions,1 transversion,1 insertion and 2 deletions.The average genetic distance of haplotypes is 0.031.The haplotypic diversity is 1.000.The nucleotide diversity is 0.0296.The average number of nucleotide differences is 13.127.It showed that the Tibetan antelope population has high variation in mitochondrial D-loop(sequence).
Keywords:Tibetan antelope  mitochondria  displacement-loop region  haplotype  genetic diversity
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